Textbook in PDF format

This detailed volume explores recent developments in microfluidics technologies for cancer diagnosis and monitoring. The book is divided into two sections that delve into techniques for liquid biopsy for cancer diagnosis and platforms for precision oncology or personalized medicine in order to create effective patient avatars for testing anti-cancer drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microfluidic Systems for Cancer Diagnosis serves as an ideal guide that will be helpful to either replicate the construction of microfluidic devices specifically developed for cancer diagnosis or to catalyze development of new and better cancer diagnostic devices.
Lateral Filter Array Microfluidic Devices for Detecting Circulating Tumor Cells
Circulating Tumor Cell Cluster Sorting by Size and Asymmetry
Digital Microfluidics with an On-Chip Drug Dispenser for Single or Combinational Drug Screening
Affinity-Based Microfluidics Combined with Atomic Force Microscopy for Isolation and Nanomechanical Characterization​ of Circulating Tumor Cells
Capture and Selective Release of Viable Circulating Tumor Cells
Single-Response Electronic Tongue and Machine Learning Enable the Multideterminati​on of Extracellular Vesicle Biomarkers for Cancer Diagnostics Without Recognition Elements
Functional Interrogation of Ca2+ Signals in Human Cancer Cells In Vitro and Ex Vivo by Fluorescent Microscopy and Molecular Tools
Microfluidic Protocols for the Assessment of Anticancer Therapies in 3D Tumor-Stromal Cocultures
A Microfluidic Approach for Enrichment and Single-Cell Characterization​ of Circulating Tumor Cells from Peripheral Blood
Rapid On-Site Evaluation (ROSE):​ A Microfluidic Approach
Microfluidic Acoustic Method for High Yield Extraction of Cell-Free DNA in Low-Volume Plasma Samples
Isolation of Extracellular Vesicles by a Microfluidic Platform to Diagnose and Monitor Pancreatic Cancer
High-Throughput Separation and Enrichment of Rare Malignant Tumor Cells from Large-Volume Effusions by Inertial Microfluidics
SAIF:​ Label-Free Separation of Circulating Tumor Cells Using a Self-Amplified Inertial Focusing Microfluidic Chip
Patient-Specific Microfluidic Cancer Spheroid Cultures for Testing Cancer Therapies
Isolation of Cancer Cells from Liquid Biopsies Using 3D-Printed Affinity Devices
A Microfluidic SERS Assay to Characterize the Phenotypic Heterogeneity in Cancer-Derived Small Extracellular Vesicles
Cluster-Wells:​ A Technology for Routine and Rapid Isolation of Extremely Rare Circulating Tumor Cell Clusters from Unprocessed Whole Blood
Secretion Function Analysis of Ex Vivo Immune Cells in an Integrated Microfluidic Device
Dynamic Tumor Perfusion and Real-Time Monitoring in a Multiplexed 3D Printed Microdevice
Capture and Release of Cancer Cells Through Smart Bioelectronics
Fabrication of Multilayer Microfluidic Arrays for Passive, Efficient DNA Trapping and Profiling