Textbook in PDF format

This collection provides methods and techniques employed when culturing, manipulating, quantifying, and functionally assessing retinal ganglion cells (RGCs), vital for better understanding various ocular diseases and for developing novel therapies. The book features exhaustive and detailed protocols for the study of RGCs at both the in vitro and in vivo level, including the culture of rodent and human cells, and immunohistochemical, morphological, and functional assessments of various in vivo models of RGC death. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Retinal Ganglion Cells: Methods and Protocols serves as an ideal guide to these important cellular features of the central nervous system and the many traumatic and degenerative blinding diseases that affect them.
Isolation and Culture of Primary Retinal Ganglion Cells from Rodent Retina
Purification of Retinal Ganglion Cells from Differentiation Through Adult via Immunopanning and Low-Pressure Flow Cytometry
Preparation of Retinal Explant Cultures
Gene Gun DiOlistic Labelling of Retinal Ganglion Cells
Differentiation of Human Embryonic/​Induced-Pluripotent Stem Cells to Retinal Ganglion Cells
Laser-Induced Ocular Hypertension in a Mouse Model of Glaucoma
Silicone Oil-Induced Ocular Hypertension Glaucoma Model (SOHU) in Rodent and Nonhuman Primate
Microbead-Induced Ocular Hypertension in a Rodent Model of Glaucoma
Viral Vector-Induced Ocular Hypertension in Mice
Optic Nerve Crush Injury in Rodents to Study Retinal Ganglion Cell Neuroprotection and Regeneration
Optical Coherence Tomography:​ Imaging Visual System Structures in Mice
Confocal Scanning Laser Ophthalmoscopy to Image Retinal Ganglion Cells in Real-Time
In Vivo Detection of Retinal Ganglion Cell Stress in Rodents with DARC
Measuring the Full-Field Electroretinogra​m in Rodents
Evaluation of Retinal Ganglion Cell via Visual Evoked Potential
High-Throughput Binocular Pattern Electroretinogra​ms in the Mouse
Intravitreal Injection of AAV for the Transduction of Mouse Retinal Ganglion Cells
Methods to Identify Rat and Mouse Retinal Ganglion Cells in Retinal Flat-Mounts